Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Flow chart of the derivation and function analysis of stress associated endoplasmic reticulum protein 1 (SERP1). (A) Flow chart. (B) Heat map of differentially expressed mRNAs in pancreatic ductal adenocarcinoma (PDAC) from GEO profiles database. The 10 mRNAs with the most significant difference are shown.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques:

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Expression level of the stress associated endoplasmic reticulum protein 1 (SERP1) in pancreatic ductal adenocarcinoma (PDAC) tissues is upregulated. (A) SERP1 was upregulated in six pairs of PDAC tissues compared with adjacent normal tissues. (B) The expression of SERP1 in eight pairs of PDAC tissues was upregulated compared with adjacent normal tissues from GEO database. (C and D) The Human Protein Atlas database results revealed that the expression level of SERP1 were upregulated in PDAC tissues compared with normal tissues. (E) Amplification of SERP1 was found in various tumors, including PDAC.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing, Amplification

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Correlation between expression level of the stress associated endoplasmic reticulum protein 1 (SERP1) and progression of pancreatic ductal adenocarcinoma (PDAC) patients. (A and B) The expression level of SERP1 in T3/4 stage PDAC specimen was significantly higher than in T1/2 stage, while there was no significant difference between SERP1 and N stage. (C) Correlation analysis was performed between expression level of SERP1 and clinical stages. SERP1 was correspondingly increased with the increase of clinical AJCC stage of PDAC. (D) Constituent ratio with high level of SERP1 was drastically different between normal samples and I/II or III/IV stage of PDAC patients.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: The correlation between expression level of the stress associated endoplasmic reticulum protein 1 (SERP1) and prognosis of pancreatic ductal adenocarcinoma (PDAC) patients. (A) K-M curve for OS of patients with high (n=89) and low (n=87) SERP1 expression level. Results revealed that high SERP1 expression level was correlated with shorter OS for PDAC patients. (B) SERP1 high expression group (n=61) had shorter DFS than SERP1 low expression group (n=76). (C) The expression of SERP1 in OS-good and poor patients. (D) The expression of SERP1 in DFS-good and poor patients. (E and F) Multivariate Cox regression analyses showed SERP1 expression was an independent factor of patients' overall survival and disease-free survival.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Downregulated stress associated endoplasmic reticulum protein 1 (SERP1) promotes cell apoptosis via regulating SRP receptor β subunit (SRPRB) associated NF-κB activation. (A) NF-κB signaling pathway gene sets were enriched in the tumor group. (B) Correlation analysis revealed that the expression level of SERP1 positively correlated with NF-κB expression in 178 pancreatic ductal adenocarcinoma (PDAC) patients from TCGA database. (C) The expression level of SRPRB was negatively correlated with NF-κB expression in 178 PDAC patients from TCGA database. (D) Western blotting confirmed that downregulated SERP1 could suppress the expression of NF-κB and the phosphorylation NF-κB in PANC-1 cells.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Activation Assay, Expressing, Western Blot, Phospho-proteomics

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Allogeneic tumor lysate can serve as both antigen source and protein supplementation for dendritic cell culture

doi: 10.1007/s00262-007-0422-0

Figure Lengend Snippet: Intracellular IFN-γ-production and cytotoxic activity of T-cells stimulated with FBS-DC or TuLy-DC. a Freshly isolated PBMC from MTC patients undergoing immunotherapy were co-cultured either with mature autologous FBS-DC or TuLy-DC. After 24 h of incubation, PBMC were stained and analyzed by flow cytometry. Percentages of CD3+-, CD4+- and CD8+T-cells positive for IFN-γ are indicated in the plots. b Freshly isolated CD3+T-cells cells were co-cultured either with (filled rectangle) mature autologous tumor lysate-pulsed FBS-DC or (open circle)TuLy-DC. CD3+T cells cultured without DC (filled triangle) or unloaded FBS-DC (open triangle) served as controls. After 6 days of incubation, cytotoxic activity of T cells against SHER-I, PANC-1 (pancreatic cancer cell line) and K-562 (chronic myelogenous leukemia cells) was evaluated using a 4-h europium release assay

Article Snippet: Before preparation of lysate SHER-I cells were extensively washed and cultured in the same medium as before but without supplementation of FCS for at least 48 h. PANC-1 and K-562 cell lines (LGC Promochem—ATCC, Teddington, UK) were cultured in RPMI 1640 medium + l -glutamine (Gibco) supplemented with 10% FBS and 50 μg/ml gentamycin in a 5% CO 2 humidified atmosphere at 37°C.

Techniques: Activity Assay, Isolation, Cell Culture, Incubation, Staining, Flow Cytometry, Release Assay